论文标题
扫描斜面显微镜中倾斜（IN）方差的几何形状
Geometry of tilt (in)variance in scanned oblique plane microscopy
论文作者
论文摘要
倾斜平面显微镜（OPM）是单个客观的灯表显微镜，通过对生成的灯表的轴向扫描执行三维（3D）成像。最近，出现了多种用于OPM中生成的灯表侧扫描的技术。但是，尚未评估它们对几何无变形的3D成像的适用性，基本上需要持续的倾斜扫描，但尚未评估。在这项工作中，我们使用几何光学方法，并得出分析关系，以实现基于平面镜面扫描的倾斜平面照明（SOPI）排列中倾斜方差的量。我们通过实验验证了派生的关系，并使用它来达到优化的扫描仪几何形状并了解其相关的局限性。我们还讨论了在优化的，倾斜的，横向扫描OPM系统中扫描对光学畸变和3D视野的影响。
Oblique plane microscopy (OPM) is a single objective light-sheet microscopy which performs three dimensional (3D) imaging by axial scan of the generated light-sheet. Recently, multiple techniques for lateral scan of the generated light-sheet in OPM have emerged. However, their suitability for geometrically distortion free 3D imaging, which essentially requires a constant tilt light-sheet scan, has not been evaluated. In this work, we use a geometrical optics approach and derive analytical relationship for the amount of tilt variance in planar mirror based scanned oblique plane illumination (SOPi) arrangement. We experimentally validate the derived relationship and use it to arrive at an optimized scanner geometry and to understand its associated limitations. We also discuss the effects of scanning on optical aberrations and 3D field of view in optimized, tilt invariant, lateral scanning OPM systems.
