论文标题
平面空的光束灯页，用于两光子显微镜
A planar Airy beam light-sheet for two-photon microscopy
论文作者
论文摘要
我们演示了第一个平面灯页面显微镜。荧光灯场显微镜已成为研究具有细胞或亚细胞分辨率的大型生物样品的首选方法。传播不变的通风梁可通过单光励元素激发观察场增加十倍。然而，灯页的特征不对称限制了其多光子激发的潜力。在这里，我们展示了如何从弯曲的传播不变的空气梁中形成平面灯页。所得的对称光板在不需要反卷积的情况下均匀地横坐在扩展的视野上均匀地激发了两光子荧光。我们演示了大量神经元组织快速两光子成像的方法。
We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a ten-fold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.
