论文标题
正常的上皮和三阴性乳腺癌细胞在刚性空间限制中显示出相同的浸润潜力
Normal epithelial and triple-negative breast cancer cells show the same invasion potential in rigid spatial confinement
论文作者
论文摘要
细胞外微环境在肿瘤生长和进展中具有基本作用，严重影响了转移性侵袭过程中单个癌细胞采用的迁移策略。在这项研究中，我们使用一种新型的微流体装置来研究间质和上皮乳腺肿瘤细胞通过趋化刺激后微结构变窄的微结构而迁移和迁移的能力。我们比较了两种间充质乳腺癌细胞系和一条上皮细胞系的迁移行为，并发现上皮细胞能够作为更侵入性的间充质细胞作为最狭窄的微收缩迁移。此外，我们证明，在没有趋化性刺激的情况下，可以发生上皮细胞通过高压环境的迁移，从而证明它们很容易反应机械线索，例如入侵细胞。
The extra-cellular microenvironment has a fundamental role in tumor growth and progression, strongly affecting the migration strategies adopted by single cancer cells during metastatic invasion. In this study, we use a novel microfluidic device to investigate the ability of mesenchymal and epithelial breast tumor cells to fluidize and migrate through narrowing microstructures upon chemoattractant stimulation. We compare the migration behavior of two mesenchymal breast cancer cell lines and one epithelial cell line, and find that the epithelial cells are able to migrate through the narrowest microconstrictions as the more invasive mesenchymal cells. In addition, we demonstrate that migration of epithelial cells through a highly compressive environment can occur in absence of a chemoattractive stimulus, thus evidencing that they are just as prone to react to mechanical cues as invasive cells.
