论文标题
高分辨率4D获取自由游泳人类精子细胞而无需染色
High-resolution 4D acquisition of freely swimming human sperm cells without staining
论文作者
论文摘要
我们提出了一种新的采集方法，该方法可以实现高分辨率，细节的全面重建，对单个人类精子细胞的三维运动和结构自由游泳。我们既可以检索精子头的三维折射率谱图，从而揭示了其良好的内部细胞器和随时间变化的方向，以及精子细胞的薄，高度动态的鞭毛的详细四维定位。使用高速偏外全息系统在自由游泳过程中获得活的人类精子细胞，该系统不需要任何移动的元素或细胞染色。重建仅基于精子细胞的自然运动和一组新型算法，从而实现了详细的四维恢复。使用这种折射率成像方法，我们认为我们已经检测到归因于中心的区域。该方法具有生物学测定和完整精子细胞的临床使用的巨大潜力。
We present a new acquisition method that enables high-resolution, fine-detail full reconstruction of the three-dimensional movement and structure of individual human sperm cells swimming freely. We achieve both retrieval of the three-dimensional refractive-index profile of the sperm head, revealing its fine internal organelles and time-varying orientation, and the detailed four-dimensional localization of the thin, highly-dynamic flagellum of the sperm cell. Live human sperm cells were acquired during free swim using a high-speed off-axis holographic system that does not require any moving elements or cell staining. The reconstruction is based solely on the natural movement of the sperm cell and a novel set of algorithms, enabling the detailed four-dimensional recovery. Using this refractive-index imaging approach, we believe we have detected an area in the cell that is attributed to the centriole. This method has great potential for both biological assays and clinical use of intact sperm cells.
