论文标题
剪切应力减少对内皮细胞的影响：肌动蛋白细胞骨架的微流体研究
The effect of shear stress reduction on endothelial cells: a microfluidic study of the actin cytoskeleton
论文作者
论文摘要
减少的血液流量是在缺血或暴露于微重力的情况下（例如在太空飞行中遇到的）导致的血液流动，从而诱导形成血管内部部分的内皮细胞所感受到的剪切应力水平的降低。在本研究中，我们使用芯片上的微脉管系统，以便在体外研究剪切应力对剪切适应的内皮细胞的影响。我们发现，在释放减少壁剪应力的一小时内，人脐静脉内皮细胞会经过其肌动蛋白骨骼的重组，减少了应激纤维的数量，肌动蛋白被募集到细胞的周围带中，表明由于流动而改变了细胞的现象型，在细胞的现象型中发生了相当快的变化。
Reduced blood flow, as occurring in ischemia or resulting from exposure to microgravity such as encountered in space flights, induces a decrease in the level of shear stress sensed by the endothelial cells forming the inner part of blood vessels. In the present study, we use a microvasculature-on-a-chip device in order to investigate in vitro the effect of such a reduction in shear stress on shear-adapted endothelial cells. We find that, within one hour of exposition to reduced wall shear stress, human umbilical vein endothelial cells undergo a reorganization of their actin skeleton, with a decrease in the number of stress fibers and actin being recruited into the cells' peripheral band, indicating a fairly fast change in cells' phenotype due to altered flow.
