论文标题
朝向纳米机电光热定位显微镜
Towards nanoelectromechanical photothermal localization microscopy
论文作者
论文摘要
单分子显微镜已成为生化分析必不可少的工具。在没有平均值的情况下表征单个分子的不同特性的能力为我们提供了对现有科学问题和现象的不同观点。最近，超分辨率荧光显微镜技术通过分子位置的定位来克服光学衍射极限。但是，标记过程可以潜在地修改分子间动力学。基于先前报道的高度敏感的纳米力学光热显微镜，我们提出了对这种无标记显微镜技术的优化，用于定位显微镜。通过金纳米颗粒实现了3 $ \ unicode {x212b} $的定位精度，并证明了依赖偏振依赖性吸收的检测，这为极化调制成像提供了进一步改进的门。
Single-molecule microscopy has become an indispensable tool for biochemical analysis. The capability of characterizing distinct properties of individual molecules without averaging has provided us with a different perspective for the existing scientific issues and phenomena. Recently, the super-resolution fluorescence microscopy techniques have overcome the optical diffraction limit by the localization of molecule positions. However, the labelling process can potentially modify the intermolecular dynamics. Based on the highly-sensitive nanomechanical photothermal microscopy reported previously, we propose optimizations on this label-free microscopy technique towards localization microscopy. A localization precision of 3 $\unicode{x212B}$ is achieved with gold nanoparticles, and the detection of polarization-dependent absorption is demonstrated, which opens the door for further improvement with polarization modulation imaging.
