论文标题
通过纤维成像束的内联全息显微镜
Inline holographic microscopy through fiber imaging bundles
论文作者
论文摘要
纤维成像束被广泛用作微型和内窥镜显微镜的薄，被动图像导管，特别是用于共聚焦荧光成像。通过纤维束的全息显微镜更具挑战性。相结合方法很复杂，需要进行广泛的校准。该文章描述了如何使用来自多模纤维的部分相干照明源通过成像束进行简单的内向全息显微镜。样品是在传输中成像的，并用捆绑包采样强度全息图并传输到远程相机。然后可以将全息图重新聚焦于体积成像，在1 mm的深度范围内达到约6 um的分辨率。该方案不需要任何复杂的事先校准，因此对弯曲不敏感。
Fiber imaging bundles are widely used as thin, passive image conduits for miniaturised and endoscopic microscopy, particularly for confocal fluorescence imaging. Holographic microscopy through fiber bundles is more challenging; phase conjugation approaches are complex and require extensive calibration. This articles describes how simple inline holographic microscopy can be performed through an imaging bundle using a partially coherent illumination source from a multimode fiber. The sample is imaged in transmission, with the intensity hologram sampled by the bundle and transmitted to a remote camera. The hologram can then be numerically refocused for volumetric imaging, achieving a resolution of approximately 6 um over a depth range of 1 mm. The scheme does not require any complex prior calibration and hence is insensitive to bending.
