论文标题
生物分子前浓缩的基于周期性浓度极化的形成，以增强生物传感
Periodic Concentration-Polarization-Based Formation of a Biomolecule Preconcentrate for Enhanced Biosensing
论文作者
论文摘要
离子浓度极化（CP）基于基本分子预浓缩是一种增强靶性生物分子检测灵敏度的已建立方法。然而，形成的预鉴定的生物分子插头迅速扫过表面弹性化的抗体，从而导致捕获剂和分析物之间的短期重叠，并随后次优的结合。为了克服这一点，我们设计了一种设置，允许通过激活CP来定期形成预估计的生物分子插头，以确定为ON/OFF间隔。这项工作证明了循环CP致动的可行性，并优化了在所需的感应区域上获得预浓缩插头最大保留时间并增强检测灵敏度所需的最大保留时间。这种方法有效地预召唤不同的分析物并成功提高免疫测定敏感性的能力强调了其在为临床和食品测试行业提供服务的免疫测定中的潜力。
Ionic concentration-polarization (CP)-based biomolecule preconcentration is an established method for enhancing the detection sensitivity of target biomolecules. However, the formed preconcentrated biomolecule plug rapidly sweeps over the surface-immobilized antibodies, resulting in a short-term overlap between the capture agent and the analyte, and subsequently suboptimal binding. To overcome this, we designed a setup allowing for periodic formation of a preconcentrated biomolecule plug by activating the CP for predetermined on/off intervals. This work demonstrated the feasibility of the cyclic CP actuation and optimized the sweeping conditions required to obtain maximum retention time of a preconcentrated plug over a desired sensing region and enhanced detection sensitivity. The ability of this method to efficiently preconcentrate different analytes and to successfully increase immunoassay sensitivity, underscore its potential in immunoassays serving the clinical and food testing industries.
