论文标题
超螺旋通过减少螺纹和纠缠来增强DNA迁移率
Supercoiling Enhances DNA Mobility by Reducing Threadings and Entanglements
论文作者
论文摘要
由于其精致的多功能性和可设计性，DNA越来越多地用于生物和纳米技术。它的大部分用途仅限于线性化和扭转放松的DNA，但是非平凡的结构以及扭转的构造或超螺旋形式的DNA质粒在很大程度上被忽略了。这部分是由于对超统在一起如何影响纠缠DNA的流变学的理解有限。为了解决这个开放的问题，我们对以扭曲链建模的DNA质粒的纠缠溶液进行大规模分子动力学（MD）模拟。我们发现，与文献中通常假设的相反，较大的超统在一起增加了纠缠制度中质粒的平均大小。同时，我们发现这伴随着意外的扩散率增加。我们解释我们的发现是由于质量间螺纹和纠缠的减少
DNA is increasingly employed for bio and nanotechnology thanks to its exquisite versatility and designability. Most of its use is limited to linearised and torsionally relaxed DNA but non-trivial architectures and torsionally constrained -- or supercoiled -- DNA plasmids are largely neglected; this is partly due to the limited understanding of how supercoiling affects the rheology of entangled DNA. To address this open question we perform large scale Molecular Dynamics (MD) simulations of entangled solutions of DNA plasmids modelled as twistable chains. We discover that, contrarily to what generally assumed in the literature, larger supercoiling increases the average size of plasmids in the entangled regime. At the same time, we discover that this is accompanied by an unexpected increase of diffusivity. We explain our findings as due to a decrease in inter-plasmids threadings and entanglement
