论文标题
单纳米颗粒的宽场可解码的正交指纹解锁多重数字测定
Wide-field Decodable Orthogonal Fingerprints of Single Nanoparticles Unlock Multiplexed Digital Assays
论文作者
论文摘要
单纳米颗粒的光学均匀性控制并调整其在正交尺寸（DOT到DOT）中的多样性，这是解锁纳米科学和应用的关键。在这里，我们报告说，可以通过上转换方案（包括界面能量迁移，浓度依赖性，能量依赖性，浓度依赖性和表面淬火者的隔离）来任意调整来自激发状态人群的上升，衰减和峰值的时间域的发射曲线，包括激发状态种群的上升，衰减和峰值力矩（T2谱）。这使我们能够显着提高纳米级的编码能力。我们进一步实施了时间分辨的宽视野成像和深度学习技术来解码这些指纹，在高吞吐量下显示出高精度。这些高维的光学指纹为从子划分限制数据存储，安全inks到高通量单分子数字测定和超级分辨率成像的应用提供了新的视野。
The control in optical uniformity of single nanoparticles and tuning their diversity in orthogonal dimensions, dot to dot, holds the key to unlock nanoscience and applications. Here we report that the time-domain emissive profile from single upconversion nanoparticle, including the rising, decay and peak moment of the excited state population (T2 profile), can be arbitrarily tuned by upconversion schemes, including interfacial energy migration, concentration dependency, energy transfer, and isolation of surface quenchers. This allows us to significantly increase the coding capacity at the nanoscale. We further implement both time-resolved wide-field imaging and deep-learning techniques to decode these fingerprints, showing high accuracies at high throughput. These high-dimensional optical fingerprints provide a new horizon for applications spanning from sub-diffraction-limit data storage, security inks, to high-throughput single-molecule digital assays and super-resolution imaging.
