论文标题
时局局域光谱：一种基于阻抗的方法，用于悬浮液中的生物细胞
Time-Constant-Domain Spectroscopy: An Impedance-based Method for Sensing Biological Cells in Suspension
论文作者
论文摘要
阻抗测量是表征和检测生物细胞电性能的常见技术。但是，要解码潜在的物理过程，它需要复杂的电气模型以及研究样本的先验知识。在这项工作中，我们基于电阻抗的测量和松弛时间（DRT）模型的测量，引入了一种无吸引力的无标签方法，用于在悬架中传感生物细胞。 DRT地图阻抗数据从频域到时间稳定域谱（TCDS）是一种有用且坚固的数据分析方法。我们在从1 kHz到1 MHz的范围内执行阻抗测量，以获取用于感测模拟样品以及悬浮液中HELA细胞的TCD。结果表明，TCD可以看作是样品的电气指纹，因为它可以用高灵敏度和分辨率来解码有关组成和结构的有用信息。
Impedance measurement is a common technique to characterize and detect the electrical properties of biological cells. However, to decode the underlying physical processes, it requires complex electrical models alongside prior knowledge of the sample under study. In this work, we introduce an attractive label-free method for sensing biological cells in suspension based on the measurement of electrical impedance and the distribution of relaxation times (DRT) model. The DRT maps impedance data from the frequency-domain to a time-constant-domain spectrum (TCDS) being a useful and robust method for data analysis. We perform impedance measurements in the range from 1 kHz to 1 MHz to obtain the TCDS for sensing mimic samples as well as HeLa cells in suspension. Results show that the TCDS can be seen as an electrical fingerprint for the sample, as it can decode useful information about the composition and structure with high sensitivity and resolution.
