论文标题
蝙蝠冠状病毒RMYN02的特征是S1/S2交界处的6-核苷酸缺失，其声称的PAA插入非常令人怀疑
The bat coronavirus RmYN02 is characterized by a 6-nucleotide deletion at the S1/S2 junction, and its claimed PAA insertion is highly doubtful
论文作者
论文摘要
周等人。报道了发现与SARS-COV-2密切相关的菌株RMYN02的发现，该菌株据称在尖峰蛋白的S1/S2连接处含有天然的PAA氨基酸插入，在PRRA插入的相同位置，该插入的位置在SARS-COV-2中产生了多基质的脂肪蛋白裂解位点。作者支持他们的发现：SARS-COV-2中存在的Furin裂解位点插入是自然的。由于Zhou等人提供了编码所假定插入区域编码的区域编码的核苷酸对齐，因此我们已经应用了几种比对算法来搜索最简约的比对。我们得出的结论是，与核苷酸水平的最接近的亲属相比，RMYN02不包含在S1/S2连接处的插入，而是6-核苷酸缺失，并且声称的PAA插入更可能是突变的结果。对RMYN02测序记录和组装方法的仔细检查是一厢情愿的。总之，SARS-COV-2及其在S1/S2连接处的12-核苷酸插入在其SARBECOVIRUS亲属中仍然是独一无二的。
Zhou et al. reported the discovery of RmYN02, a strain closely related to SARS-CoV-2, which is claimed to contain a natural PAA amino acid insertion at the S1/S2 junction of the spike protein at the same position of the PRRA insertion that has created a polybasic furin cleavage site in SARS-CoV-2. The authors support with their findings the theory that the furin cleavage site insertion present in SARS-CoV-2 is natural. Because no nucleotide alignment with closely related strains of the region coding for the supposed insertion is provided by Zhou et al., we have applied several alignment algorithms to search for the most parsimonious alignments. We conclude that RmYN02 does not contain an insertion at the S1/S2 junction when compared to its closest relatives at the nucleotide level, but rather a 6-nucleotide deletion and that the claimed PAA insertion is more likely to be the result of mutations. A close examination of RmYN02 sequencing records and assembly methods is wishful. In conclusion, SARS-CoV-2, with its 12-nucleotide insertion at the S1/S2 junction remains unique among its sarbecovirus relatives.
