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We initiate the study of diameter computation in geometric intersection graphs from the fine-grained complexity perspective. A geometric intersection graph is a graph whose vertices correspond to some shapes in $d$-dimensional Euclidean space, such as balls, segments, or hypercubes, and whose edges correspond to pairs of intersecting shapes. The diameter of a graph is the largest distance realized by a pair of vertices in the graph. Computing the diameter in near-quadratic time is possible in several classes of intersection graphs [Chan and Skrepetos 2019], but it is not at all clear if these algorithms are optimal, especially since in the related class of planar graphs the diameter can be computed in $\widetilde{\mathcal{O}}(n^{5/3})$ time [Cabello 2019, Gawrychowski et al. 2021]. In this work we (conditionally) rule out sub-quadratic algorithms in several classes of intersection graphs, i.e., algorithms of running time $\mathcal{O}(n^{2-δ})$ for some $δ>0$. In particular, there are no sub-quadratic algorithms already for fat objects in small dimensions: unit balls in $\mathbb{R}^3$ or congruent equilateral triangles in $\mathbb{R}^2$. For unit segments and congruent equilateral triangles, we can even rule out strong sub-quadratic approximations already in $\mathbb{R}^2$. It seems that the hardness of approximation may also depend on dimensionality: for axis-parallel unit hypercubes in~$\mathbb{R}^{12}$, distinguishing between diameter 2 and 3 needs quadratic time (ruling out $(3/2-\varepsilon)$- approximations), whereas for axis-parallel unit squares, we give an algorithm that distinguishes between diameter $2$ and $3$ in near-linear time. Note that many of our lower bounds match the best known algorithms up to sub-polynomial factors.