论文标题
使用延长的源Shack-Hartmann波前传感器，单发定量像差和散射长度测量
Single-shot quantitative aberration and scattering length measurements in mouse brain tissues using an extended-source Shack-Hartmann wavefront sensor
论文作者
论文摘要
哺乳动物脑组织中的深层荧光成像由于散射和光学异常引起的信号和分辨率损失而保持挑战。使用自适应光学（AO）校正像差需要在组织中可靠的测量。在这里，我们表明，扩展的源棚屋 - hartmann波前传感器（ESSH）允许通过固定的脑切片进行定量的像差测量，其厚度最大为四倍的散射长度。我们特别证明，基于图像相关性的这种波前测量方法与基于标准的质心方法相比，散射更强大。最后，我们利用了棚屋 - 哈特曼传感器的几何形状来获得组织散射长度的测量。
Deep fluorescence imaging in mammalian brain tissues remains challenging due to scattering and optical aberration-induced loss in signal and resolution. Correction of aberrations using adaptive optics (AO) requires their reliable measurement in the tissues. Here, we show that an extended-source Shack-Hartmann wavefront sensor (ESSH) allows quantitative aberration measurements through fixed brain slices with a thickness up to four times their scattering length. We demonstrate in particular that this wavefront measurement method based on image correlation is more robust to scattering compared to the standard centroid-based approach. Finally, we obtain a measurement of the tissue scattering length taking advantage of the geometry of a Shack-Hartmann sensor.
