论文标题
键选择强度衍射断层扫描
Bond-Selective Intensity Diffraction Tomography
论文作者
论文摘要
在广泛使用的全息图和计算成像技术中，恢复分子信息仍然是一个巨大的挑战。为了应对这一挑战，我们开发了一种计算中国光热显微镜，称为键选择强度衍射断层扫描（BS-IDT）。 BS-IDT基于具有附加脉冲光源的低成本Brightfield显微镜，从仅强度测量值中恢复了红外光谱和键选择性的3D折射率图。高保真红外指纹光谱提取已验证。生物细胞的体积化学成像以每体积约20秒的速度证明，横向和轴向分辨率分别为〜350 nm和〜1.1微米。 BS-IDT的应用潜力是通过对癌细胞中储存在癌细胞中的脂质和秀丽隐杆线虫上的体积化学成像（约100微米x 100微米）上的化学化学成像进行了研究的。
Recovering molecular information remains a grand challenge in the widely used holographic and computational imaging technologies. To address this challenge, we developed a computational mid-infrared photothermal microscope, termed Bond-selective Intensity Diffraction Tomography (BS-IDT). Based on a low-cost brightfield microscope with an add-on pulsed light source, BS-IDT recovers both infrared spectra and bond-selective 3D refractive index maps from intensity-only measurements. High-fidelity infrared fingerprint spectra extraction is validated. Volumetric chemical imaging of biological cells is demonstrated at a speed of ~20 seconds per volume, with a lateral and axial resolution of ~350 nm and ~1.1 micron, respectively. BS-IDT's application potential is investigated by chemically quantifying lipids stored in cancer cells and volumetric chemical imaging on Caenorhabditis elegans with a large field of view (~100 micron X 100 micron).
