论文标题
生物粒子静态光散射的测量
Measurement of static light scattering of bio-particles
论文作者
论文摘要
小颗粒的光散射是主要是主要是生物起源的颗粒系统的特性,是最普遍,最非侵入性的技术之一。由于它们在生物学和生物医学科学中的重要性,因此选择了包括生物粒子在内的亚微米颗粒。通过使用一种原始设计和制造的极性和依赖性的光依赖性光散射设置,在两个不同的入射光波长下进行了同质,致病性假单胞菌和非致病性分枝杆菌的光散射研究。通过使用T-Matrix方法来验证我们的分析来生成理论散射图。还使用新型的蒙特卡洛模拟技术进行了这些颗粒的光散射模拟。本文介绍了实验,理论和模拟结果之间的关系。我们研究了铜绿假单胞菌和分枝杆菌,并试图通过使用光散射工具来观察其形态学特性。
Light scattering by small particles is one of the most prevailing and non-invasive technique for examining the properties of particulate systems chiefly of biological origin. The sub-micron particles including the bio-particles were so chosen because of their importance in biology and biomedical sciences. Light scattering investigation from homogenous, pathogenic Pseudomonas aeruginosa and non-pathogenic Mycobacterium smegmatis was carried out at two different wavelength of incident light, by using an original designed and fabricated polar and azimuth-dependent light scattering setup. Theoretical scattering plots were generated by using T-matrix approach to validate our analyses. Simulations of light scattering of these particles were also carried out using a novel Monte- Carlo simulation technique. The relation between experimental, theoretical and simulated result is presented in this paper. We have studied Pseudomonas aeruginosa and Mycobacterium smegmatis, and attempted for prospect of observing its morphological property by using light scattering tool.