论文标题
折射率在生物颗粒的光散射测量中的影响
Influence of refractive index in light scattering measurements of biological particles
论文作者
论文摘要
可以通过非侵入性和非破坏性分析方法对各种颗粒物进行调查。使用此工具对小颗粒进行探索必须准确地解决折射率的概念。光散射的解释结果,用于定量病毒和细菌细胞等生物学颗粒形态的形态,只能通过其折射率的精确证据来验证。许多研究人员较早地报道了通过从散射光中收集信息的大小和形状定量,但是像折射率这样的参数被认为是从文献中采用的标准值或被选为同样生物学颗粒的中位数。在包含适当的折射率值以启示光散射结果时需要进行强有力的分析。否则,在前报告的类似作品中,这种重要的看法并不强烈。我们主要尝试在光散射研究中找到折射率的影响,并涉及对生物颗粒,例如金黄色葡萄球菌,大肠杆菌,Corona病毒(SARS COV 2)等。
Investigations on diverse particulate matter can be carried out by non-invasive and non-destructive analytical means of light scattering technique. Exploration of small particles using this tool must address the concept of refractive index accurately. Interpretation of light scattering results for quantification of morphology of biological particles like viruses and bacterial cells can only be validated by exact evidence of its refractive index. Size and shape quantification of such particles by collecting information from the scattered light, was reported earlier by many researchers but parameter like refractive index was considered as a standard value employed from literature or selected as a median value of biological particles which are alike. A strong analysis is needed on inclusion of appropriate value of refractive index for revelation of light scattering results. This important perception is otherwise not considered strongly in similar works reported earlier. We primarily took an attempt to find the influence of refractive index in light scattering studies with allusion to biological particles like Staphylococcus aureus, Escherichia coli, Corona virus (SARS COV 2) etc.