论文标题
具有多个中间体和途径的单分子的力依赖性折叠动力学
Force-Dependent Folding Kinetics of Single Molecules with Multiple Intermediates and Pathways
论文作者
论文摘要
大多数单分子研究都从均衡跳舞实验中得出了天然，中间和展开状态的动力学速率。在这里，我们应用Kramers动力学扩散模型来从非平衡拉力实验中得出中间状态的力依赖性动力学速率。从动力学速率中，我们还提取了力依赖性动力学屏障和平衡折叠能。我们将方法应用于具有多个折叠途径和中间体的DNA发夹。实验结果与理论预测一致。此外，提出的非平衡单分子方法使我们能够表征天然，展开和中间状态的动力学和热力学特性，这些特性无法从平衡跳舞实验中得出。
Most single-molecule studies derive the kinetic rates of native, intermediate, and unfolded states from equilibrium hopping experiments. Here, we apply Kramers kinetic diffusive model to derive the force-dependent kinetic rates of intermediate states from non-equilibrium pulling experiments. From the kinetic rates, we also extract the force-dependent kinetic barriers and the equilibrium folding energies. We apply our method to DNA hairpins with multiple folding pathways and intermediates. The experimental results agree with theoretical predictions. Furthermore, the proposed non-equilibrium single-molecule approach permits us to characterize kinetic and thermodynamic properties of native, unfolded, and intermediate states that cannot be derived from equilibrium hopping experiments.
