论文标题
谷氨酰胺合成酶（GS）的结构和功能的生物信息学分析
Bioinformatic analysis for structure and function of Glutamine synthetase(GS)
论文作者
论文摘要
目的：预测假偏蛋白酶sp的谷氨酰胺合成酶（GS）的结构和功能。通过生物信息学技术，并为进一步研究提供了理论基础。方法：pseudoalteromonas sp的GS序列的开放阅读框（ORF）。由ORF查找器获得，并转化为氨基酸残基。通过BLAST分析结构域。通过分析工具的方法：Protparam，Protscale，SignalP-4.0，TMHMM，Sopma，Swiss-Model，NCBI Smart-Blast和Maga 7.0，预测和分析了蛋白质的结构和功能。结果：结果表明该序列是具有468个氨基酸残基的GS，理论分子量为51986.64 Da。该蛋白质与Shewanella Oneidensis具有最接近的进化状态。然后它没有信号肽位点和跨膜结构域。 GS的二级结构包含35.04％的α-螺旋，16.67％的延伸链，5.34％β-转移，42.95％Rantancil。结论：此GU是蛋白质的多种生物学功能，可以用作极端环境中微生物氮代谢的分子样本。
Objective: To predict structure and function of Glutamine synthetase (GS) from Pseudoalteromonas sp. by bioinformatics technology, and to provide a theoretical basis for further study. Methods: Open reading frame (ORF) of GS sequence from Pseudoalteromonas sp. was obtained by ORF finder and was translated into amino acid residue. The structure domain was analyzed by Blast. By the method of analysis tools: Protparam, ProtScale, SignalP-4.0, TMHMM, SOPMA, SWISS-MODEL, NCBI SMART-BLAST and MAGA 7.0, the structure and function of the protein were predicted and analyzed. Results: The results showed that the sequence was GS with 468 amino acid residues, theoretical molecular weight was 51986.64 Da. The protein has the closest evolutionary status with Shewanella oneidensis. Then it had no signal peptide site and transmembrane domain. Secondary structure of GS contained 35.04% alpha-helix, 16.67% Extended chain, 5.34% beta-turn, 42.95% RandomCoil. Conclusions: This GU was a variety of biological functions of protein that may be used as a molecular samples of microbial nitrogen metabolism in extreme environments.
