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In recent years, reinforcement learning has faced several challenges in the multi-agent domain, such as the credit assignment issue. Value function factorization emerges as a promising way to handle the credit assignment issue under the centralized training with decentralized execution (CTDE) paradigm. However, existing value function factorization methods cannot deal with ad-hoc cooperation, that is, adapting to new configurations of teammates at test time. Specifically, these methods do not explicitly utilize the relationship between agents and cannot adapt to different sizes of inputs. To address these limitations, we propose a novel method, called Relation-Aware Credit Assignment (RACA), which achieves zero-shot generalization in ad-hoc cooperation scenarios. RACA takes advantage of a graph-based relation encoder to encode the topological structure between agents. Furthermore, RACA utilizes an attention-based observation abstraction mechanism that can generalize to an arbitrary number of teammates with a fixed number of parameters. Experiments demonstrate that our method outperforms baseline methods on the StarCraftII micromanagement benchmark and ad-hoc cooperation scenarios.