论文标题
冷冻电子显微镜中密度图的三维比对
Three-Dimensional Alignment of Density Maps in Cryo-Electron Microscopy
论文作者
论文摘要
冷冻电子显微镜（Cryo-EM）数据处理中的一个常见任务是比较大分子的三维密度图。在本文中，我们提出了一种用于对齐三维密度图的算法，该算法利用了地图投影图像之间的共同线路。该算法是完全自动的，并且处理旋转，反射（递手）以及地图之间的翻译。另外，该算法适用于任何类型的分子对称性，而无需任何有关地图对称性的信息。我们在公开可用的密度图上评估了对齐算法的准确性和效率。该算法可从https://github.com/shkolniskylab/emalign获得。
A common task in cryo-electron microscopy (cryo-EM) data processing is to compare three-dimensional density maps of macromolecules. In this paper, we propose an algorithm for aligning three-dimensional density maps that exploits common lines between projection images of the maps. The algorithm is fully automatic and handles rotations, reflections (handedness), and translations between the maps. In addition, the algorithm is applicable to any type of molecular symmetry without requiring any information regarding the symmetry of the maps. We evaluate our alignment algorithm on publicly available density maps, demonstrating its accuracy and efficiency. The algorithm is available at https://github.com/ShkolniskyLab/emalign.
