论文标题
NIO/CUO纳米复合材料和NiO纳米纤维作为非酶电化学胆固醇生物传感器的评估和比较
Evaluation and comparison of NiO/CuO nanocomposite and NiO nanofibers as non-enzymatic electrochemical cholesterol biosensor
论文作者
论文摘要
由于其低氧化还原活性，用于检测胆固醇的高度敏感的非酶电化学传感器以检测胆固醇是一个具有挑战性的问题。在本研究中，已经探索了金属氧化物的新型组合的电化学响应。通过静电纺丝法制造NiO/CuO纳米复合材料，并使用扫描电子显微镜，EDX和X射线衍射技术研究了其形态和结构特性。电化学研究表明，NIO/CUO/GC电极在中性缓冲液溶液中呈线性检测范围在0.8至6.5 mm之间，低检测极限为5.9 microM，灵敏度为10.27 microA.mm-1 .cm-2。制造的电极的响应时间为3秒，并且具有良好的区分干扰物种的能力。此外，纳米复合电极表现出较高的稳定性以及其他有利因素，使其成为检测胆固醇的有效传感器。此外，循环伏安法实验表明，NIO/GCE的当前响应约为NiO/CuO/GCE响应的30％，这表明纳米复合材料比Nio纳米纤维具有更大的电催化活性对胆固醇氧化。 NIO/GCE结果显示了2.5 microa.mm-1 .cm-2， 响应时间为2秒，在三周时间内的稳定性有限。
Fabrication of a highly sensitive non-enzymatic electrochemical sensor for the detection of cholesterol is a challenging issue due to its low redox activity. In the present study, the electrochemical response of a novel combination of metal oxides has been explored. NiO/CuO nanocomposite was fabricated by electrospinning method and its morphology and structural characteristics were studied using scanning electron microscopy, EDX and X-ray diffraction techniques. Electrochemical studies revealed that the NiO/CuO/GC electrode poses a linear detection range between 0.8 to 6.5 mM, low detection limit of 5.9 microM and a sensitivity of 10.27 microA.mM-1 .cm-2 in the neutral buffer solution. The fabricated electrode has a response time of 3 seconds and a good ability to discriminate interfering species. Moreover, the nanocomposite electrode exhibited high stability as well as the other favorable factors, makes it an effective sensor for the detection of cholesterol. In addition, cyclic voltammetry experiments showed that the current response of NiO/GCE is approximately 30 % of the NiO/CuO/GCE response, suggesting that the nanocomposite has a greater electrocatalytic activity towards cholesterol oxidation than NiO nanofibers. NiO/GCE results showed the sensitivity of 2.5 microA.mM-1 .cm-2 , response time of 2 seconds and a limited stability over three-week time span.
