DB43/T2697—2023 43 湖 南 省 地 方 标 准ICS CCS 97.195 Y 88 发 布 湖南省市场监督管理局紫甘薯脱毒苗高效繁育技术规程 2023-08发布-22 2023-11实施-22code of efficient breeding of virus-free seedings of purple sweet potato DB43/T 2697—2023 I 目 次 前言 ································ ································ ································ ·························· Ⅲ 1 范围 ································ ································ ································ ······················ 1 2 规范性引用文件 ································ ································ ································ ······· 1 3 术语和定义 ································ ································ ································ ············· 1 4 脱毒培养 ································ ································ ································ ················ 1 4.1 品种选择 ································ ································ ································ ········ 1 4.2 催芽 ································ ································ ································ ··············· 2 4.3 消毒 ································ ································ ································ ··············· 2 4.4 茎尖接种培养与病毒检测 ································ ································ ··················· 2 5 脱毒苗繁育 ································ ································ ································ ············· 3 5.1 脱毒苗扩繁 ································ ································ ································ ····· 3 5.2 苗圃繁育 ································ ································ ································ ········ 3 6 种苗繁育 ································ ································ ································ ················ 3 6.1 原原种生产 ································ ································ ································ ····· 3 6.2 原种生产 ································ ································ ································ ········ 3 6.3 脱毒种苗繁育 ································ ································ ································ ·· 3 7 病虫害防治 ································ ································ ································ ············· 3 8 生产档案 ································ ································ ································ ················ 4 附录A（资料性）甘薯主要病虫害防治表 ································ ································ ··········· 5 附录B（资料性）生产档案记载表 ································ ································ ···················· 6 DB43/T 2697—2023 II DB43/T 2697—2023 III 前 言 本文件按照 GB/T 1.1—2020《标准化工作导则 第1部分：标准化文件的结构和起草规则》的规 定起草。 请注意本文件的某些内容可能涉及专利。本文件的发布机构不承担识别专利的责任。 本文件由湖南省农业农村厅提出。 本文件由湖南省农业标准化技术委员会归口。 本文件起草单位：湖南省棉花科学研究所、湖南应用技术学院、安乡县农业农村局、澧县农业农村 局、汉寿聚农种养专业合作社 本文件主要起草人：巩养仓、李锋，陈泽南、朱春生、冯俊利、李庠、马杰、李玉芳、傅淋、贺璐、 刘芳、李偲、肖才升、何顺、赵伶俐、曾良。 DB43/T 2697—2023 IV DB43/T 2697—2023 1 紫甘薯脱 毒苗高效繁育技术规程 1 范围 本文件规定了紫甘薯脱毒苗繁育的脱毒培养、脱毒苗繁育、种苗繁育、病虫害防治等技术要求。 本文件适用于紫甘薯脱毒种苗的繁育。 2 规范性引用文件 下列文件中的内容通过文中的规范性引用而构成本文件必不可少的条款。 其中， 注日期的引用文件， 仅该日期对应的版本适用于本文件；不注日期的引用文件，其最新版本（包括所有的修改单）适用于本 文件。 GB 7413 甘薯种苗产地检疫规程 GB/T 8321 农药合理使用准则 NY/T 402 脱毒甘薯种薯（苗）病毒检测技术规程 NY/T 496 肥料合理使用通则 NY/T 1200 甘薯脱毒种薯 HNZ 175 甘薯脱毒种薯繁育技术规程 3 术语和定义 下列术语和定义适用于本文件。 3.1 紫甘薯脱毒组培苗 virus -free seedings of purple sweet potato 指应用紫甘薯茎尖分生组织培养技术获得的再生组培苗，经检测确认不带甘薯羽状斑驳病毒 （SPFMV） 、甘薯潜隐病毒（ SPLV）和甘薯（ SPVD）病毒，在容器内繁殖的种苗。 3.2 紫甘薯原原种 breeder’s seed of purple sweet potato 指利用脱毒紫甘薯组培苗在符合 NY/T 1200 标准要求的防蚜虫网棚或温室生产的一代脱毒种薯。 3.3 紫甘薯原种 original seed of purple sweet potato 指利用紫甘薯原原种在隔离条件好的防蚜虫网棚生产出的二代脱毒种薯。 4 脱毒培养 4.1 品种选择 选择通过国家或省审（认）定（登记）的适宜于本生态区种植、花青素含量高的紫甘薯品种。在留 种田块挑选具有本品种特征特性，茎叶生长健壮，叶片无叶斑、无皱缩、无花叶、无卷叶等病症表现的 DB43/T 2697—2023 2 薯块。 4.2 催芽 用清水清洗 薯块，湿润纱布覆盖，置于 32 ℃培养箱中催芽，待芽长到 2 cm以上使用。 4.3 消毒 4.3.1 实验室 应提前一周对实验室进行全面空间消毒，每 1 m3用40％